The cell concentration was then diluted and added to each tube. Concentration of Vitamin D were made in separate tube with concentrations of 0% (control), 1%, 5% and 10%. This represents a cell density of approximately 10⁸ cells/mL. The culture was placed in an incubator (37☌) until a density of 50 Klett spectrophotometer units was reached. epidermidisĩ Hypotheses Null Hypothesis – the Vitamin D will have no significant effect on the survivorship of UV stressed staph cells Alternate Hypothesis – the Vitamin D will have a significant effect on the survivorship of UV stressed staph cellsġ0 Materials Sterile Dilution Fluid (200mM KH2O4, 100nM K2HPO4, 10mM MgSO4, 10nM MgSO4, 1mM NaCl) Sterile test tubes Sterile spreader bars Staphylococcus epidermidis Incubator Ethanol Bunsen burner Vortex LB agar plates (0.5% yeast extract, 1% tryptone, 1% sodium chloride) Vitamin D (liquid supplement) Micropipettes Sterile tips Klett spectrophotometer Labeling tape Lab Conoco UVC hood (254nm UVC cm2 at working surface)ġ1 Procedure Bacteria (Staph) was grown overnight in sterile LB Media.Ī sample of the overnight culture was added to fresh media in a sterile sidearm flask. Liver and kidney damage Hypercalcemia – buildup of calcium in the bloodstreamĨ Purpose The purpose of this experiment is to determine if vitamin D will significantly lower the effects UV radiation has on S. Humans-sunburn, skin cancer, sun stroke FDA approved protection – sunscreen, hats, sunglasses, anti-radiation clothingĦ Vitamin D Fat-soluble secosteroids Bone growthĮasily synthesized by the body Uncertain effects Measured in IUs, about 4,000 IUs per mLħ Toxicity of Vitamin D Hypervitaminosis D Excess Vitamin D Increase in free radical production Increased risk of cancer Cell degenerationĤ Ultra Violet Light Radiation from the sun Most stopped by the ozone layer Shorter wavelength than visible=more powerful 100nm-380nm Gram positive Non-pathogenic Common surface symbiont Forms biofilms Presentation on theme: "UV Light Effects on Vitamin D Stressed Staph Cells"- Presentation transcript:ġ UV Light Effects on Vitamin D Stressed Staph CellsĪdam Parrish Central Catholic High School Grade 11
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